Active Recombinant Human KLK1, His-tagged

• Cat.No.: KLK1-742H
• Product Overview: Recombinant Human KLK1 (Accession # NP_002248) Ile25-Ser262, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 25-262 a.a.
• Predicted N Terminal: Val23 (KLK-5 propeptide, Accession # Q9Y337)
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and CaCl2.
• Bio-activity: Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human KLK1, His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 41 kDa, 45 kDa, 49 kDa and 56 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: KLK1 kallikrein 1 [ Homo sapiens ]
• Official Symbol: KLK1
• Synonyms: KLK1; kallikrein 1; kallikrein 1, renal/pancreas/salivary; kallikrein-1; Klk6; tissue kallikrein; glandular kallikrein 1; kallikrein serine protease 1; kidney/pancreas/salivary gland kallikrein; hK1; KLKR
• Gene ID: 3816
• mRNA Refseq: NM_002257
• Protein Refseq: NP_002248
• UniProt ID: P06870
• Chromosome Location: 19q13.3