| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Description : |
Kallikreins are members of a highly conserved serine proteases that are involved in the post-translational modification of many polypeptides, and plays a role in diverse physiological processes. Fifteen kallikreins who seen coding genes are located in a cluster on chromosome 19 have been reported, and growing evidence suggests that many kallikreins are implicated in carcinogens is and some have potential as novel cancer and other disease biomakers. Human kallikrein1(KLK1), also known as tissue kallikrein, is functionally conserved in its capacity to cleave the low molecular weight kininogen to release the vasoactive peptide, Lys-bradykinin which plays a role in regulating vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation. |
| Form : |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Bio-activity : |
Fully biologically active when compared to standard. The specific activity determined by cleaving a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFRAMC) is no less than 1,500 pmoles/min/μg. |
| Molecular Mass : |
29.7 kDa |
| AA Sequence : |
MWFLVLCLAL SLGGTGAAPP IQSRIVGGWE CEQHSQPWQA ALYHFSTFQC GGILVHRQWV LTAAHCISDN YQLWLGRHNL FDDENTAQFV HVSESFPHPG FNMSLLENHT RQADEDYSHD LMLLRLTEPA DTITDAVKVV ELPTEEPEVG STCLASGWGS IEPENFSFPD DLQCVDLKIL PNDECKKAHV QKVTDFMLCV GHLEGGKDTC VGDSGGPLMC DGVLQGVTSW G |
| Endotoxin : |
Less than 1 EU/μg of rHuKLK-1, His as determined by LAL method. |
| Purity : |
>90% by SDS-PAGE and HPLC analyses. |
| Usage : |
This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at< -20="" centigrade.="" further="" dilutions="" should="" be="" made="" in="" appropriate="" buffered="" solutions.=""> |
