Active Recombinant Human Kallikrein-Related Peptidase 11

• Cat.No.: KLK11-1360H
• Product Overview: KLK11 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain having a molecular weight of 25.6kDa though on SDS-PAGE migrates at about 41kDa due to the glycosilation. The KLK11 is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: KLK11 is a multifunctional protease. KLK11 cleaves "bz-Phe-Arg-4-methylcoumaryl-7-amide", KLK11 is a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. KLK11 cleaves synthetic peptides after arginine rather than lysine residues.
• Form: The KLK11 protein was lyophilized from a 0.2µm filtered solution containing no additives or preservatives.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Bio-activity: KLK11 specific activity is greater than 2200 pmoles/min/μg when measure with 100uM colormetric peptide substrate (D-Val-Leu-Lys-ThioBenzyl ester) and 1 μg of activated enzyme. The reaction is carried out in a volume of 100μL containing 50mM Tris, 1M NaCl, 10mM EDTA, 0.1mM DTNB (5,5μDithio-bis(2-nitrobenzoic acid)), pH8.5 at 37°C. Cleavage of the peptide substrate can be measured at a wavelength of 405nm and quantified using the extinction coeffiecient 13,260M-1cm-1.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized KLK11 in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: ETRIIKGFEC KPHSQPWQAA LFEKTRLLCG ATLIAPRWLL TAAHCLKPRY IVHLGQHNLQ KEEGCEQTRT ATESFPHPGF NNSLPNKDHR NDIMLVKMAS PVSITWAVRP LTLSSRCVTA GTSCLISGWG STSSPQLRLP HTLRCANITI IEHQKCENAY PGNITDTMVC ASVQEGGKDS CQGDSGGPLV CNQSLQGIIS WGQDPCAITR KPGVYTKVCK YVDWIQETMK NN.
• Storage: Lyophilized KLK11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KLK11 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: KLK11 kallikrein-related peptidase 11 [ Homo sapiens ]
• Official Symbol: KLK11
• Synonyms: kallikrein-related peptidase 11; TLSP; PRSS20; MGC33060; KLK11; hK11; kallikrein 11; hippostasin; protease, serine, trypsin-like; protease, serine, 20 trypsin-like; EC 3.4.21.-; Hippostasin; hippostasin; Serine protease 20; Trypsin-like protease; kallikrein-related peptidase 11
• Gene ID: 11012
• mRNA Refseq: NM_001136032
• Protein Refseq: NP_001129504
• MIM: 604434
• UniProt ID: Q9UBX7
• Chromosome Location: 19q13.3-q13.4
• Function: serine-type endopeptidase activity; peptidase activity