Active Recombinant Human Jun Oncogene

• Cat.No.: JUN-1027H

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Protein Length: 1-331 aa
• Description: C-Jun is the name of a gene and protein which, in combination with c-Fos, forms the AP-1 early response transcription factor. It was first identified as the Fos-binding protein p39 and only later rediscovered as the product of the c-jun gene. It is activated through double phosphorylation by the JNK pathway but has also a phosphorylation-independent function. C-Jun knockout is lethal, but transgenic animals with a mutated c-Jun that cannot be phosphorylated (termed c-JunAA) can survive.The growth- promoting activity of c-jun is mediated by repression of tumor suppressors, as well as up-regulation of positive cell cycle regulators. Mostly, c-jun is a positive regulator of cell proliferation, whereas JunB has the converse effect. Recombinant c-jun was expressed in aBaculovirussystem and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein contains no detectable protease, DNase, and RNase activity.
• Form: Liquid. Supplied in 20mM Tris-HCl pH8.0, 20% glycerol, 100mM KCl, 0.2mM EDTA and 1mM DTT.
• Activity: 100ng are sufficient for a protein-protein interaction assay.
• Application: C-fos has been applied in protein-DNA and protein-protein interaction assays.
• Purity: >90% by SDS-PAGE.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months. Store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: JUN jun oncogene [ Homo sapiens ]
• Synonyms: JUN; jun oncogene; AP1; AP-1; c-Jun; activator protein 1; enhancer-binding protein AP1; Jun activation domain binding protein; v-jun sarcoma virus 17 oncogene homolog; v-jun avian sarcoma virus 17 oncogene homolog
• Gene ID: 3725
• mRNA Refseq: NM_002228
• Protein Refseq: NP_002219
• MIM: 165160
• UniProt ID: P05412
• Chromosome Location: 1p32-p31
• Pathway: B cell receptor signaling pathway; Colorectal cancer; Epithelial cell signaling in Helicobacter pylori infection; ErbB signaling pathway; Focal adhesion; GnRH signaling pathway; MAPK signaling pathway; Neurotrophin signaling pathway; Pathways in cancer; Renal cell carcinoma; T cell receptor signaling pathway; Toll-like receptor signaling pathway; Wnt signaling pathway; Signaling in Immune system
• Function: R-SMAD binding; RNA polymerase II transcription factor activity; Rho GTPase activator activity; double-stranded DNA binding; promoter binding; protein dimerization activity; transcription factor activity; transcription factor binding