Active Recombinant Human ITGA8&ITGB1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: ITGA8&ITGB1-1433H
• Product Overview: Biotinylated Recombinant Human ITGA8&ITGB1 protein(Phe39-Leu1012(ITGA8)&Gln21-Asp728(ITGB1)), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 39-1012;21-728 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA8 & ITGB1 Heterodimer Protein with a linear range of 0.078-2.5 μg/mL.
• Molecular Mass: Biotinylated Human ITGA8&ITGB1 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGA8 and ITGB1, has a calculated MW of 116.4 kDa (ITGA8) and 83.7 kDa (ITGB1). Subunit ITGA8 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-150 kDa (ITGA8) and 100-115 kDa (ITGB1) respectively due to glycosylation.
• Proteinlength: Phe39-Leu1012(ITGA8)&Gln21-Asp728(ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA8 integrin, alpha 8 [ Homo sapiens ]
• Official Symbol: ITGA8
• Synonyms: ITGA8; integrin, alpha 8; integrin alpha-8
• Gene ID: 8516
• mRNA Refseq: NM_003638
• Protein Refseq: NP_003629
• MIM: 604063
• UniProt ID: P53708