Active Recombinant Human ITGA8 & ITGB1 Heterodimer Protein, His & Avi-tagged, Biotinylated

• Cat.No.: ITGA8&ITGB1-362H
• Product Overview: Recombinant Human ITGA8 & ITGB1 Heterodimer Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Phe 39 - Leu 1012 (ITGA8) & Gln 21 - Asp 728 (ITGB1) (Accession # P53708-1 (ITGA8) & P05556-1 (ITGB1)). Predicted N-terminus: Phe 39 (ITGA8) & Gln 21 (ITGB1). Biotinylated Human ITGA8 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA8 and ITGB1, has a calculated MW of 116.4 kDa (ITGA8) & 83.7 kDa (ITGB1). Subunit ITGA8 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 110 kDa and 120 kDa due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Avi
• ProteinLength: Phe 39 - Leu 1012 (ITGA8) & Gln 21 - Asp 728 (ITGB1)
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Fibronectin at 5 μg/mL (100 μL/well) can bind Recombinant Human ITGA8 & ITGB1 Heterodimer Protein, His & Avi-tagged, Biotinylated with a linear range of 0.156-5 μg/mL (QC tested).
• Molecular Mass: 116.4 kDa (ITGA8) & 83.7 kDa (ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA8 integrin, alpha 8 [ Homo sapiens ] & ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) [ Homo sapiens ]
• Official Symbol: ITGA8 & ITGB1
• Synonyms: ITGA8; integrin, alpha 8; integrin alpha-8; & ITGB1; FNRB, MDF2, MSK12; integrin beta-1; CD29; GPIIA; FNRB; MDF2; VLAB; MSK12; VLA-BETA
• Gene ID: 8516 & 3688
• mRNA Refseq: NM_003638 & NM_002211
• Protein Refseq: NP_003629 & NP_002202
• MIM: 604063 & 135630
• UniProt ID: P53708 & P05556