Active Recombinant Human ITGA5 & ITGB1 Protein, His-Avi-tagged, Biotinylated

• Cat.No.: ITGA5 & ITGB1-1877H
• Product Overview: Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein is expressed from HEK293 cells. It contains AA Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) (Accession # P08648-1 (ITGA5) & P05556-1 (ITGB1)). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 42-995;21-728 a.a.
• Predicted N Terminal: Phe 42 (ITGA5) & Gln 21 (ITGB1)
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human Fibronectin at 5 ug/mL (100 uL/well) can bind Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein with a linear range of 2-78 ug/mL.
• Molecular Mass: Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 112.5 kDa (ITGA5) & 83.7 kDa (ITGB1). The reducing protein migrates as 120-130KDa (ITGA5) and 100-115 kDa (ITGB1) respectively due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: ITGA5; ITGB1
• Synonyms: Integrin alpha 5 beta 1, ITGA5 & ITGB1