Active Recombinant Human Interleukin 36 Receptor Antagonist

• Cat.No.: IL36RN-5660H
• Product Overview: RecombinantHuman IL36RN protein was expressed in E. coli. Val2-Asp155.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 2-155 a.a.
• Description: IL36RN isa member of the IL-1 family of proteins. IL-1 family members include IL-1β,IL-1α, IL-1ra, IL-18 and IL-1F5-F10.All family members show a 12 β-strand,β-trefoil configuration, and all family members are believed to have arisenfrom a common ancestral gene that underwent multiple duplications. The human IL-36Ra/IL-1F5gene is in closest proximity to the gene for IL-1ra and is likely arelatively recent duplication of the IL-1ra gene.
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Val2
• MolecularWeight: 17 kDa
• Activity: Measuredby its ability to inhibit IL-36α, IL-36β or IL-36γ-induced IL-8 secretion by humanpreadipocytes. The ED50 for this effect is typically 0.25-1.25 μg/mL in thepresence of 20 ng/mL of recombinant human IL-36β. Measured by its bindingability in a functional ELISA. Immobilized recombinant human IL-36Ra at 1μg/mL (100 μL/well) can bind recombinant human IL-1 Rrp2 Fc Chimera with alinear range of 0.15-5 μg/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 65 kDa,reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: IL36RN interleukin 36 receptorantagonist [ Homo sapiens ]
• Official Symbol: IL36RN
• Synonyms: IL36RN; interleukin36 receptor antagonist; FIL1; FIL1D; IL1F5; IL1L1; PSORP; IL1HY1;IL1RP3; IL36RA; FIL1 (DELTA); interleukin-36 receptor antagonist protein; IL-1rahomolog 1; interleukin-1 HY1; IL-1 related protein 3; interleukin-1-likeprotein 1; family of interleukin 1-delta; IL1F5 (Canonical product IL-1F5a);interleukin 1 family, member 5 (delta); interleukin-1 receptor antagonisthomolog 1; IL-1F5 (IL-1HY1, FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta)
• Gene ID: 26525
• mRNA Refseq: NM_012275
• Protein Refseq: NP_036407
• MIM: 605507
• UniProt ID: Q9UBH0
• Chromosome Location: 2q14
• Function: cytokine activity;interleukin-1 receptor antagonist activity