Active Recombinant Human Interleukin 34, His-tagged

• Cat.No.: IL34-652H
• Product Overview: RecombinantHuman IL34 protein was expressed in Murine myeloma cell line. Asn21-Pro242,with a C-terminal 6-His tag.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 21-242 a.a.
• Description: IL34 issecreted as a homodimer consisting of 39 kDa monomers. It belongs to no knowncytokine family. Human IL34 is synthesized as a242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222aa mature chain. The mature chain contains one potential site of N-linkedglycosylation. Human IL34 is 71% identicaltomouse IL34 on theamino acid level. IL34 is expressed in various tissues, includingthe heart, brain, liver, kidney, spleen, thymus, testes, ovary, smallintestine, prostate, and colon, and is most abundant in the spleen.
• Form: Lyophilized from a0.2 um filtered solution in PBS and NaCl with BSA as a carrier protein.
• N-terminalSequence: Asn21
• MolecularWeight: 26.1 kDa
• Activity: Measuredin a cell proliferation assay using human peripheral blood monocytes. The ED 50for this effect is typically 0.75-3.75 ng/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 35-38kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months,-20to-70°C under sterile conditions after opening.

Gene Information

• Gene Name: IL34 interleukin 34 [ Homosapiens ]
• Official Symbol: IL34
• Synonyms: IL34; interleukin 34; IL-34; C16orf77; interleukin-34; chromosome 16 open reading frame 77; MGC34647
• Gene ID: 146433
• mRNA Refseq: NM_001172771
• Protein Refseq: NP_001166242
• MIM: 612081
• UniProt ID: Q6ZMJ4
• Chromosome Location: 16q22.1
• Function: cytokine activity;growth factor activity; macrophage colony-stimulating factor receptor binding