Active Recombinant Human Interleukin 3 (Colony-stimulating Factor, Multiple)

• Cat.No.: IL3-120H
• Product Overview: Recombinant Human interleukin 3 (colony-stimulating factor, multiple) protein sequence (containing the signal peptide sequence, and the mature human Interleukin 3 sequence) was expressed in modifiedhuman 293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Interleukin-3 (IL-3) is a pleiotropic cytokine that regulates the proliferation, maturation, and survival of progenitor cells of the myeloid, erythroid, and megakaryocyte lineage. IL-3 is predominantly produced by activated T cells although expression has also been detected in monocytes and macrophages, NK-cells, mast cells, endothelial cells, and keratinocytes.
• Amino Acid Sequence: APMTQTTSLKTS WVNCSNMID EIITHLKQPPL PLLDFNNLN GEDQDILME NNL RRP NLEA FNRAVKSLQN ASAIESILKNLL PCLPLATAAPTRH PIHIKDGDWNE FRRKLTFYLKTLENAQAQQTTLSLAIF.
• Molecular Mass: IL-3 migrates as a broad band between 15 and 25 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL-3 that has a predicted molecular mass of 15.1 kDa.
• PI: IL-3 separates into a number of isoforms with a pI between 4.2 and 6.8 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-3 that has a predicted pI of 7.05.
• % Carbohydrate: Purified IL-3 consists of 0-40% carbohydrate by weight.
• Glycosylation: IL-3 has N-linked and possibly O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Product should be stored at 2 to 8°C. Short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
• Activity: The ED₅₀of IL-3 is typically 0.1- 0.4 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line.

Gene Information

• Gene Name: IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ]
• Synonyms: IL3; interleukin 3 (colony-stimulating factor, multiple); IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF; P-cell stimulating factor; hematopoietic growth factor; interleukin 3; mast-cell growth factor; multilineage-colony -stimulating factor; MULTI-CSF; Hematopoietic growth factor; P-cell-stimulating factor; Mast cell growth factor; Multipotential colony-stimulating factor
• Gene ID: 3562
• mRNA Refseq: NM_000588
• Protein Refseq: NP_000579
• MIM: 147740
• UniProt ID: P08700
• Chromosome Location: 5q31.1
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Asthma; Hematopoietic cell lineage; Fc epsilon RI signaling pathway
• Function: interleukin-3 receptor activity; cytokine activity; growth factor activity; protein binding