Active Recombinant Human Interleukin 18

Cat.No. : IL18-433H
Product Overview : Interleukin-18 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 18302 Dalton. The IL-18 is purified by proprietary chromatographic techniques.
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Species : Human
Source : Human
Tag : Non
Description : IL-18 is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.
Form : Lyophilized from a concentrated (1mg/ml) solution in water containing 0.5x PBS pH=7.3.
Purity : Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bio-activity : The ED50 as determined by the dose-dependant stimulation of the IFN-g by human PBMC co-stimulated with IL-12 was found to be 5ng/ml corresponding to a Specific Activity of 200,000IU/mg.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility : It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage : Lyophilized Interleukin 18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL18 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Pathways : African trypanosomiasis; Cellular roles of Anthrax toxin; Cytokine Signaling in Immune system; Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway; Hypertrophy Model; IL12 signaling mediated by STAT4; IL12-mediated signaling events; Immune System; Influenza A; Interleukin-1 processing; Malaria; NOD-like receptor signaling pathway; Rheumatoid arthritis; Signaling by Interleukins; Tuberculosis
Gene Name IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ]
Official Symbol IL18
Synonyms Interferon-gamma-inducing factor; IGIF; IL-1g; IL-18; IL1F4; MGC12320; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; Iboctadekin; IL18
Gene ID 3606
mRNA Refseq NM_001562
Protein Refseq NP_001553
MIM 600953
UniProt ID Q14116
Chromosome Location 11q22.2-q22.3
Function cytokine activity; signal transducer activity

Not For Human Consumption!

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What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy) 04/26/2023

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.

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