Active Recombinant Human Interleukin 17A

• Cat.No.: IL17A-487H
• Product Overview: Recombinant Human IL-17A produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 264 amino acids and having a molecular mass of 30,250 Dalton.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells.
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 98% as determined by: Reducing and non-reducing SDS-PAGE. H-IL-17-A consists of monomer and dimer at an approximate ratio of 50:50.
• Bio-activity: The ED50 as determined by the dose-dependant induction of IL-6 in primary human foreskin fibroblasts was found to be 2 ng/mL, corresponding to a specific activity of 5 x 105 Units/mg.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg"s/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Reducing and non-reducing SDS-PAGE. H-IL-17-A consists of monomer and dimer at an approximate ratio of 50:50.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
• Pathways: Cytokine-cytokine receptor interaction

Gene Information

• Gene Name: 17A interleukin 17A [ Homo sapiens ]
• Official Symbol: IL17A
• Synonyms: IL17A; interleukin 17A; IL17; CTLA8; IL-17; IL-17A; toxic T-lymphocyte-associated antigen 8; otoxic T-lymphocyte-associated protein 8; cytotoxic T-lymphocyte-associated serine esterase 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• Gene ID: 3605
• mRNA Refseq: NM_002190
• Protein Refseq: NP_002181
• MIM: 603149
• UniProt ID: Q16552
• Chromosome Location: 6p12
• Function: cytokine activity