Active Recombinant Human Interleukin 1 Family, Member 6 (Epsilon)

• Cat.No.: IL1F6-398H
• Product Overview: IL1F6 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 158 amino acids and having a molecular mass of 17.7kDa. The IL1F6 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: IL1F6-398H
• Description: Human interleukin family 1, member 6 (hIL-1F6 / FIL1-epsilon) belongs to the interleukin 1 cytokine family. IL1F6 is expressed in the immune system and the fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. IL1F6 is the only IL-1 family member found to be expressed on T-cells. IL1F6 functions via receptor complex of IL-1Rrp2 and IL-1RAcP. The recombinant IL1F6, along with IL1F8 and IL1F9, acts as an agonist by activating the pathway involving NF?B and MAPK in an IL-1Rrp2 dependent manner.
• Source: Human
• Host: Escherichia Coli.
• Form: IL1F6 was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Bio-activity: As measured by its binding ability in a functional ELISA, immobilized IL1F6 at 1 µg/ml (100 µl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15- 5 µg/ml.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to quick spin followed by reconstitution of IL1F6 in PBS to a concentration no less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Leu.
• Storage: Lyophilized IL1F6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1F6 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
• Tag: Non

Gene Information

• Gene Name: IL1F6 interleukin 1 family, member 6 (epsilon) [ Homo sapiens ]
• Official Symbol: IL1F6
• Synonyms: IL1F6; interleukin 1 family, member 6 (epsilon); FIL1; FIL1E; IL-1F6; MGC129552; MGC129553; IL1(EPSILON); FIL1(EPSILON); IL-1F6 (FIL-1-epsilon); interleukin 1, epsilon; Interleukin-1 family member 6; Interleukin-1 epsilon; IL-1 epsilon; FIL1 epsilon
• Gene ID: 27179
• mRNA Refseq: NM_014440
• Protein Refseq: NP_055255
• MIM: 605509
• UniProt ID: Q9UHA7
• Chromosome Location: 2q12-q14.1
• Function: cytokine activity; interleukin-1 receptor binding