Active Recombinant Human Intercellular Adhesion Molecule 5, Telencephalin, Fc-tagged

• Cat.No.: ICAM5-5642H
• Product Overview: RecombinantHuman ICAM5 protein was expressed in Murine myeloma cell line with the Fcpart of Human IgG1 Pro100-Lys330 (C-terminus) and Human ICAM5 Ala28-Glu570(N-terminus).

Specification

• Cat. No.: ICAM5-5642H
• Description: ICAM5 isa cell surface glycoprotein belonging to the immunoglobulin superfamily.Human ICAM5 consists of an 832 amino acid (aa) extracellular domaincontaining 9 immunoglobulin (Ig) domains and 15 N-glycosylation sites, a 28aa transmembrane domain, and a 64 aa cytoplasmic domain. ICAM5 shares 38-55%aa identity with other ICAMs, being most closely related to ICAM-1 (50% identity)and ICAM-3 (55% identity). Human and mouse ICAM5 share 85% aa identity.
• Source: Mammalian cells
• Form: Lyophilizedfrom a 0.2 um filtered solution in PBS.
• N-terminalSequence: Ala28
• MolecularWeight: 85.1 kDa (monomer)
• Activity: Measuredby the ability of the immobilized protein to support the adhesion ofPMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemiacells. When 5x104 cells/well are added to rhICAM5/Fc Chimeracoated plates (25 μg/mL, 100 μL/well), approximately 25-40% will adhere after1 hour incubation at 37°C.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 125-130kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 400 μg/mLin sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.
• Protein length: 28-570 a.a.

Gene Information

• Gene Name: ICAM5intercellular adhesion molecule 5, telencephalin [ Homo sapiens ]
• Official Symbol: ICAM5
• Synonyms: ICAM5; intercellularadhesion molecule 5, telencephalin; TLN; TLCN; intercellular adhesionmolecule 5; ICAM-5; telencephalin
• Gene ID: 7087
• mRNA Refseq: NM_003259
• Protein Refseq: NP_003250
• MIM: 601852
• UniProt ID: Q60625
• Chromosome Location: 19p13.2