Active Recombinant Human IL7R Protein, His-tagged

• Cat.No.: IL7R-01H
• Product Overview: Recombinant Human IL7R Protein (Glu21-Asp239), with a C-terminal 6-His tag, was expressed in Human embryonic kidney cell.

Specification

• Description: The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found.
• Source: Human embryonic kidney cell
• Species: Human
• Tag: His
• Bio-activity: Measured by its binding ability in a functional ELISA. In a His Tag Antibody coated plate, when Recombinant Human IL-7R alpha/CD127 His-tag is present at 500 ng/mL, Recombinant Human IL-7 binds with an ED50 of 0.75-4.5 ng/mL.
• Molecular Mass: 44-51 kDa
• N-terminal Sequence Analysis: Glu21
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.
• Protein length: 21-239 a.a.

Gene Information

• Gene Name: IL7R interleukin 7 receptor [ Homo sapiens (human) ]
• Official Symbol: IL7R
• Synonyms: IL7R; interleukin 7 receptor; ILRA; CD127; IL7RA; CDW127; IL-7R-alpha; interleukin-7 receptor subunit alpha; CD127 antigen; IL-7 receptor subunit alpha; IL-7R subunit alpha; interleukin 7 receptor alpha chain
• Gene ID: 3575
• mRNA Refseq: NM_002185
• Protein Refseq: NP_002176
• MIM: 146661
• UniProt ID: P16871