Active Recombinant Human IL6 Protein (Pro29-Met212), GMP Grade, Animal-Free

• Cat.No.: IL6-24HG
• Product Overview: GMP recombinant human IL-6 protein (Pro29-Met212) without tag was expressed in E. coli, produced in an Animal-Free laboratory and manufactured and tested under cGMP guidelines.

Specification

• Species: Human
• Source: E.coli
• Protein Length: Pro29-Met212
• Description: This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2).
• Bio-activity: Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. The ED50 for this effect is 0.2-0.8 ng/mL.
  The specific activity of Recombinant Human IL-6 is >1.0 x 108 IU/mg, which is calibrated against the human IL-6 WHO International Standard.
• Molecular Mass: Predicted Molecular Mass: 20.9 kDa
  SDS-PAGE: 20-21 kDa, reducing conditions
• N-terminal Sequence Analysis: Pro29-Val-Pro-Pro-Gly-Glu-Asp-Ser-Lys-Asp
• Endotoxin: <0.10 EU/ μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Reconstitution: Reconstitute at 100-200 μg/mL in sterile PBS.
• Shipping: The product is shipped with polar packs.

Gene Information

• Gene Name: IL6 interleukin 6 (interferon, beta 2) [ Homo sapiens ]
• Official Symbol: IL6
• Synonyms: IL6; interleukin 6 (interferon, beta 2); IFNB2; interleukin-6; BSF2; HGF; HSF; IL 6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor; IL-6
• Gene ID: 3569
• mRNA Refseq: NM_000600
• Protein Refseq: NP_000591
• MIM: 147620
• UniProt ID: P05231