Active Recombinant Human IL32 Protein, His-tagged

• Cat.No.: IL32-00H
• Product Overview: Recombinant Human IL32 (Met1-Lys188)Protein with a His tag at N-terminus was expressed in E. coli.

Specification

• Description: This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: E. coli
• Species: Human
• Tag: His
• Bio-activity: Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells under serum free conditions in the presence of 10 μg/mL of Polymyxin B. Kim, S.H. et al. (2005) Immunity 22:131. The ED50 for this effect is 10-50 ng/mL.
• Molecular Mass: 23.1 kDa
• Protein length: 1-188
• N-terminal Sequence Analysis: Met
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >90% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after opening.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and CHAPS.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: IL32 interleukin 32 [ Homo sapiens (human) ]
• Official Symbol: IL32
• Synonyms: IL32; interleukin 32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma; interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cell transcript 4; natural killer cells protein 4; tumor necrosis factor alpha-inducing factor
• Gene ID: 9235
• mRNA Refseq: NM_001012631
• Protein Refseq: NP_001012649
• MIM: 606001
• UniProt ID: P24001