Active Recombinant Human IL2RB protein, His&Twin-Strep-tagged

• Cat.No.: IL2RB-7125H
• Product Overview: Recombinant Human IL2RB protein(Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG) ), fused with His and Twin-Strep tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His&Twin Strep
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human IL-2, Tag Free at 5 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag with a linear range of 20-78 ng/mL.
• Molecular Mass: Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 29.9 kDa (IL-2RB) and 34.1 kDa (IL-2RG). Subunit IL-2RB is fused with a polyhistidine tag at the C-terminus and subunit IL-2RG is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 40-44 kDa and 60-65 kDa respectively due to glycosylation.
• Protein length: Ala 27 - Asp 239 (IL-2RB) & Leu 23 - Asn 254 (IL-2RG)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL2RB interleukin 2 receptor, beta [ Homo sapiens ]
• Official Symbol: IL2RB
• Synonyms: IL2RB; interleukin 2 receptor, beta; IL15RB, interleukin 15 receptor, beta; interleukin-2 receptor subunit beta; CD122; p75; IL-2RB; CD122 antigen; IL-2R subunit beta; IL-2 receptor subunit beta; interleukin 15 receptor, beta; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta; IL15RB; P70-75
• Gene ID: 3560
• mRNA Refseq: NM_000878
• Protein Refseq: NP_000869
• MIM: 146710
• UniProt ID: P14784