Active Recombinant Human IL25 protein, hFc-tagged

• Cat.No.: IL25-148H
• Product Overview: Recombinant Human IL25 protein(NP_073626.1)(Tyr 33-Gly177), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 33-177 a.a.
• Form: Lyophilized from sterile PBS , pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human IL17BR-His at 10 μg/ml (100 μl/well) can bind human Fc-IL25, The EC50 of human Fc-IL25 is 1.7-4.2 μg/mL.2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED50 for this effect is 15-60 ng/mL.
• Molecular Mass: The recombinant human IL25/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and predicts a molecular mass of 45 KDa. As a result of glycosylation, the apparent molecular mass of rh IL25/Fc monomer is approximately 50-60 KDa in SDS-PAGE under reducing conditions.
• Proteinlength: Tyr33-Gly177
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL25 interleukin 25 [ Homo sapiens ]
• Official Symbol: IL25
• Synonyms: IL25; interleukin 25; IL17E, interleukin 17E; interleukin-25; IL 17E; IL 25; interleukin 17E; interleukin-17E; IL17E
• Gene ID: 64806
• mRNA Refseq: NM_022789
• Protein Refseq: NP_073626
• MIM: 605658
• UniProt ID: Q9H293