Active Recombinant Human IL23A&IL12B protein, His-Avi-tagged, Biotinylated

• Cat.No.: IL23A&IL12B-1613H
• Product Overview: Biotinylated Recombinant Human IL23A&IL12B protein(Arg20-Pro189(IL23A)&Ile23-Ser328(IL12B)), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His&Avi
• ProteinLength: 20-189;23-328 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Anti-p19 subunit of IL23 (Anti-IL23A) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free with a linear range of 1.2-19.5 ng/mL.Immobilized Anti-p40 subunit of IL23 (Anti-IL23B) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free with a linear range of 4.9-39 ng/mL.Immobilized Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human IL-23 R, Fc Tag with a linear range of 0.4-6 ng/mL.
• Molecular Mass: Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with an Avi tag (Avitag™), followed by a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) respectively due to glycosylation.
• Proteinlength: Arg20-Pro189(IL23A)&Ile23-Ser328(IL12B)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL23A interleukin 23, alpha subunit p19 [ Homo sapiens ]
• Official Symbol: IL23A
• Synonyms: IL23A; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; IL 23; IL 23A; IL23P19; interleukin six; G CSF related factor; P19; SGRF; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor; IL-23; IL-23A; MGC79388
• Gene ID: 51561
• mRNA Refseq: NM_016584
• Protein Refseq: NP_057668
• MIM: 605580
• UniProt ID: Q9NPF7