Active Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated

• Cat.No.: IL23A&IL12B-334H
• Product Overview: Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) (Accession # NP_057668 (IL23A) & AAH67499 (IL12B)). Predicted N-terminus: His (IL23A) & Ile 23 (IL12B). Biotinylated Human IL23A & IL12B Heterodimer Protein, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with an Avi tag, followed by a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) respectively due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-p40 subunit of IL23（Anti-IL12B） MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated with a linear range of 4.9-39 ng/mL (Routinely tested).
  Immobilized Anti-p19 subunit of IL23 (Anti-IL23A) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated with a linear range of 1.2-19.5 ng/mL (QC tested).
  Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated stimulates secretion of IL-17 by mousespleen cells. The ED50 for this effect is 0.191-0.2173ng/ml (Routinely tested).
  Cell based assay shows that the secretion of IL-17 induced by Recombinant Human IL23A & IL12B Heterodimer Protein, His & Avi-tagged, Biotinylated is inhibited by increasing concentration of the anti-human IL-23 neutralizing antibody. The IC50 is between 0.25-1.40 ng/mL (Routinely tested).
• Molecular Mass: 21.9 kDa (IL23A) & 34.7 kDa (IL12B)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: IL23A interleukin 23, alpha subunit p19 [ Homo sapiens ] & IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
• Official Symbol: IL23A & IL12B
• Synonyms: IL23A; IL 23; IL 23A; IL23P19; P19; SGRF; IL-23-A; IL-23p19; IL-23; IL-23A; MGC79388; & IL12B; NKSF2; CLMF; CLMF2; p40; IL 12B; IL12; subunit p40; NKSF; CLMF p40; IL-12B
• Gene ID: 51561 & 3593
• mRNA Refseq: NM_016584 & NM_002187
• Protein Refseq: NP_057668 & NP_002178
• MIM: 605580 & 161561
• UniProt ID: Q9NPF7 & P29460