Active Recombinant Human IL2 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: IL2-0184H
• Product Overview: Biotinylated Recombinant Human IL2 protein(Ala21-Thr153), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 21-153 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human IL-2, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human IL-2 R alpha, His Tag with a linear range of 1-39 ng/mL.Immobilized Human IL-2 R alpha, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2, Fc,Avitag with a linear range of 0.6-10 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminus and an Avi tag at the C-terminus.The protein has a calculated MW of 43.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala21-Thr153
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• UniProt ID: P60568