Active Recombinant Human IL1R1, DDDDK-tagged

• Cat.No.: IL1R1-527H
• Product Overview: Recombinant Human IL1R1 (NP_000868.1) (Met1-Thr332), fused with five amino acids (DDDDK) at the C-terminus, was produced in Human Cells.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: Met1-Thr332
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500 pg/mL IL1b. The ED50 for this effect is typically 40-200 ng/mL.
• Molecular Mass: The recombinant human IL1R1 consists of 321 amino acids and predicts a molecular mass of 36.8 KDa. It migrates as an approximately 56 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL1R1 interleukin 1 receptor, type I [ Homo sapiens ]
• Official Symbol: IL1R1
• Synonyms: IL1R1; interleukin 1 receptor, type I; IL1R, IL1RA; interleukin-1 receptor type 1; CD121A; D2S1473; IL-1R-1; IL-1RT1; IL-1RT-1; antigen CD121a; interleukin receptor 1; interleukin-1 receptor alpha; interleukin-1 receptor type I; CD121 antigen-like family member A; interleukin 1 receptor alpha, type I; P80; IL1R; IL1RA; IL-1R-alpha
• Gene ID: 3554
• mRNA Refseq: NM_000877
• Protein Refseq: NP_000868
• MIM: 147810
• UniProt ID: P14778