Active Recombinant Human IL1F5 protein

• Cat.No.: IL1F5-316H
• Product Overview: Recombinant Human IL1F5 protein(Q9UBH0)(Met1-Asp155) was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• ProteinLength: Met1-Asp155
• Form: Lyophilized from sterile PBSPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit IL36α-induced IL8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is typically 0.1-0.4μg/mL in the presence of 20ng/mL IL36α.
• Molecular Mass: The recombinant human IL1F5 consists of 156 amino acids and predicts a molecular mass of 17.1 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 99 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.