Active Recombinant Human IL18BP protein, His-tagged

• Cat.No.: IL18BP-342H
• Product Overview: Recombinant Human IL18BP protein (Met1-Gly194), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gly194
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: 1. Measured by its ability to bind biotinylated Cynomolgus IL18 in functional ELISA.
  2. Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 0.05-0.35μg/mL in the presence of 50 ng/mL of recombinant human IL-18.
• Molecular Mass: The recombinant human IL18BPa consists of 175 amino acids and has a predicted molecular mass of 19 kDa.In SDS-PAGE under reducing conditions, the apparent molecular mass of human IL18BPa is approximately 40.3 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL18BP interleukin 18 binding protein [ Homo sapiens ]
• Official Symbol: IL18BP
• Synonyms: IL18BP; interleukin 18 binding protein; interleukin-18-binding protein; IL18BPa; MC51L 53L 54L homolog gene product; IL-18BP; tadekinig-alfa; MC51L-53L-54L homolog gene product
• Gene ID: 10068
• mRNA Refseq: NM_001039659
• Protein Refseq: NP_001034748
• MIM: 604113
• UniProt ID: O95998