Active Recombinant Human IL18 (37-193aa)

• Cat.No.: IL18-335H
• Product Overview: Human IL18 (Q14116, 37 a.a.-193 a.a.) partial recombinant protein expressed in Escherichia coli.

Specification

• Cat. No.: IL18-335H
• Description: Interleukin-18 also known as IL18 is a protein which in humans is encoded by the IL18 gene. The protein encoded by this gene is a proinflammatory cytokine. IL-18 is a cytokine produced by macrophages and other cells that belongs to the IL-1 superfamily. IL-18 works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). After stimulation with IL-18, natural killer (NK) cells and certain T cells release another important cytokine called interferon-μ (IFN-μ) or type II interferon that plays an important role in activating the macrophages or other cells.
• Sequence: ENLESDYFGK LESKLSVIRN LNDQVLFIDQ GNRPLFEDMT DSDCRDNAPR TIFIISMYKD SQPRGMAVTI SVKCEKISTL SCENKIISFK EMNPPDNIKD TKSDIIFFQR SVPGHDNKMQ FESSSYEGYF LACEKERDLF KLILKKEDEL GDRSIMFTVQ NED
• Theoretical MW (kDa): 18
• Form: Liquid
• Preparation Method: Escherichia coli expression system
• Purity: > 98% by SDS-PAGE and HPLC
• Endotoxin Level: < 0.1 ng/ug (1 EU/ug)
• Activity: The ED50 was determined by the dose-dependent proliferation of human PBMC co-stimulated with IL-12 cells was found to be in the range of 5 ng/mL
• Application: SDS-PAGE
• Storage Buffer: In PBS
• Storage: Store at 4°C for 1 month or store at -20°C for 6 months. Aliquot to avoid repeated freezing and thawing.
• Unitprot ID: P01563
• Pathways: African trypanosomiasis; African trypanosomiasis; Cellular roles of Anthrax toxin; Cytokine-cytokine receptor interaction; Cytokine-cytokine receptor interaction; Cytosolic DNA-sensing pathway; Cytosolic DNA-sensing pathway; Hypertrophy Model; IL12 signaling mediated by STAT4; IL12-mediated signaling events; IL23-mediated signaling events; Influenza A; Interleukin-1 processing; Malaria; NOD-like receptor signaling pathway; Rheumatoid arthritis; Signaling by Interleukins; Signaling in Immune system; Tuberculosis
• Tag: Non

Gene Information

• Gene Name: IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ]
• Official Symbol: IL18
• Synonyms: IL18; interleukin 18 (interferon-gamma-inducing factor); IGIF; IL-18; IL-1g; IL1F4; MGC12320; interleukin-18; IL-1 gamma; iboctadekin; OTTHUMP00000234749; OTTHUMP00000234750; OTTHUMP00000234751; interleukin-1 gamma; IFN-gamma-inducing factor; Interferon gamma-inducing factor; Iboctadekin; Interleukin-1 gamma
• Gene ID: 3606
• mRNA Refseq: NM_001562
• Protein Refseq: NP_001553
• MIM: 600953
• Chromosome Location: Q14116
• Function: cytokine activity; cytokine activity; signal transducer activity

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.