Active Recombinant Human IL17F protein

• Cat.No.: IL17F-129H
• Product Overview: Recombinant Human IL17F was expressed in E. coli.

Specification

• Description: Interleukin 17F is a disulfide-linked homodimeric protein belonging to the IL-17 family of proinflammatory cytokines. Members belonging to this family share conserved features of cysteine-knot structures but differ in their N-terminal regions. The T-helper cell produced IL-17 family is inducible by IL15 and IL23, and IL17F induces T-cell and PBMC activation and proliferation. It also performs cartilage matrix turnover and angiogenesis prevention. Out of the IL-17 family, IL17F is the most homologous to IL17 and dimerizes in a way similar to that of nerve growth factors (NGF) and other neurotrophins.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)
• Bio-activity: The ED50 is 8.0-40.0ng/mL as determined by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells.
• Molecular Mass: 15.2 kDa
• Endotoxin: <1.0 EU/μg of recombinant protein as determined by the LAL method.
• Purity: >95%
• Applications: Cell Assay
• Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 centigrade. Upon reconstitution, this cytokine can be stored in working aliquots at 2 centigrade - 8 centigrade for one month, or at -20 centigrade for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
• Reconstitution: Reconstitute in sterile 4mM HCl at 25μg/mL (containing at least 0.1% human or bovine serum albumin)
• Tag: Non

Gene Information

• Gene Name: IL17F interleukin 17F [ Homo sapiens ]
• Official Symbol: IL17F
• Synonyms: IL17F; interleukin 17F; interleukin-17F; IL 17F; ML 1; ML1; IL-24; cytokine ML-1; mutant IL-17F; interleukin-24; ML-1; CANDF6; IL-17F
• Gene ID: 112744
• mRNA Refseq: NM_052872
• Protein Refseq: NP_443104
• MIM: 606496
• UniProt ID: Q96PD4
• Chromosome Location: 6p12
• Pathway: IL23-mediated signaling events, organism-specific biosystem
• Function: cytokine activity; cytokine binding; protein homodimerization activity