Active Recombinant Mouse Interleukin 17F

• Cat.No.: Il17f-497M
• Product Overview: Recombinant Mouse IL-17F produced in E.coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 133 amino acids and having a molecular mass of 14.9 kDa.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: Interleukin-17 (IL-17 or IL-17A) is the founding member of a group of cytokines called the IL-17 family. Interleukin 17 is a cytokine that acts as a potent mediator in delayed ?C type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation, similar to Interferon gamma. IL- 17 is produced by T helper cells and is induced by IL ?C 23 which results in destructive tissue damage in delayed ?C type reactions.
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: IL-17F is fully bioactive when compared to standard. The ED50 as determined by the dose-dependant induction of IL-6 in NHDF is 1.94 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: MRKNPKAGVP ALQKAGNCPP LEDNTVRVDI RIFNQNQGIS VPREFQNRSS SPWDYNITRD PHRFPSEIAEAQCRHSGCIN AQGQEDSTMN SVAIQQEILV LRREPQGCSN SFRLEKMLLK VGCTCVKPIV HQAA
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: Il17f interleukin 17F [ Mus musculus ]
• Official Symbol: Il17f
• Synonyms: Il17f; interleukin 17F; C87042; IL-17F
• Gene ID: 257630
• mRNA Refseq: NM_145856
• Protein Refseq: NP_665855
• UniProt ID: Q7TNI7
• Chromosome Location: 1 A4
• Function: cytokine activity