Active Recombinant Mouse Interleukin 17F

• Cat.No.: Il17f-497M
• Product Overview: Recombinant Mouse IL-17F produced in E.coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 133 amino acids and having a molecular mass of 14.9 kDa.

Specification

• Cat. No.: Il17f-497M
• Description: Interleukin-17 (IL-17 or IL-17A) is the founding member of a group of cytokines called the IL-17 family. Interleukin 17 is a cytokine that acts as a potent mediator in delayed ?C type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation, similar to Interferon gamma. IL- 17 is produced by T helper cells and is induced by IL ?C 23 which results in destructive tissue damage in delayed ?C type reactions.
• Species: Mouse
• Source: E.coli
• Form: Lyophilized with no additives.
• Purity: Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: IL-17F is fully bioactive when compared to standard. The ED50 as determined by the dose-dependant induction of IL-6 in NHDF is 1.94 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
• Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: MRKNPKAGVP ALQKAGNCPP LEDNTVRVDI RIFNQNQGIS VPREFQNRSS SPWDYNITRD PHRFPSEIAEAQCRHSGCIN AQGQEDSTMN SVAIQQEILV LRREPQGCSN SFRLEKMLLK VGCTCVKPIV HQAA
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
• Tag: Non

Gene Information

• Gene Name: Il17f interleukin 17F [ Mus musculus ]
• Official Symbol: Il17f
• Synonyms: Il17f; interleukin 17F; C87042; IL-17F
• Gene ID: 257630
• mRNA Refseq: NM_145856
• Protein Refseq: NP_665855
• UniProt ID: Q7TNI7
• Chromosome Location: 1 A4
• Function: cytokine activity

Reviews

11/01/2022

High specificity.

02/23/2020

Good for receptor studies.

02/05/2018

Potent in anti-bacterial assays.

Q&As

What techniques are used to assess IL17F's involvement in cancer development and its potential as a therapeutic target?

Tumor xenografts, immune cell infiltration analysis, and therapeutic antibody testing assess IL17F's role in cancer.

What methodologies are employed to explore IL17F's role in promoting neutrophil recruitment and activation at mucosal sites?

Neutrophil chemotaxis assays, in vivo neutrophil tracking, and immune cell activation analysis explore IL17F's impact on neutrophil functions.

How do researchers differentiate the functional roles of IL17F from other IL17 family members in immune responses?

Genetic knockout models, functional assays, and gene expression analyses distinguish IL17F's unique roles.

How is the interplay between IL17F and gut microbiota investigated, and what are the implications for intestinal health?

Microbiota composition analysis, germ-free models, and immune cell profiling investigate IL17F-microbiota interactions.

Could you elaborate on the strategies employed to investigate IL17F's contribution to autoimmune diseases using experimental models and patient samples?

Animal disease models, patient samples, and immunological assays elucidate IL17F's contribution to autoimmune diseases.

What experimental techniques are utilized to dissect the complex heterodimerization of IL17F with IL17A and its impact on signaling pathways?

Co-immunoprecipitation, fluorescence resonance energy transfer (FRET), and pathway-specific assays elucidate IL17F's heterodimerization and signaling.

How do researchers investigate the potential synergistic or antagonistic effects of IL17F on tissue inflammation in comparison to other cytokines?

Comparative cytokine profiling, inflammatory assays, and pathway analysis dissect IL17F's interplay with other immune mediators.

What molecular approaches are used to study IL17F's involvement in chronic inflammatory conditions, such as asthma and inflammatory bowel diseases?

Allergen challenges, inflammatory disease models, and gene expression profiling probe IL17F's involvement in chronic conditions.

Can you explain the experimental designs used to study the impact of IL17F on barrier function in epithelial tissues?

Epithelial barrier permeability assays, tissue section analysis, and cell culture models study IL17F's effects on barrier integrity.

How do researchers explore the effects of IL17F modulation on tissue repair and regeneration in experimental models?

Wound healing assays, tissue regeneration models, and histological analyses examine IL17F's effects on tissue repair.