Active Recombinant Human IL12RB2 Protein, Fc/His-tagged

• Cat.No.: IL12RB2-9483H
• Product Overview: Recombinant Human IL-12 R beta 2 protein (Lys24-Asn622) with Human IgG1 (Pro100-Lys330) and a 6-His tag at C-terminus was expressed in mouse myeloma cell line.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc&His
• Protein Length: 24-622 a.a.
• Description: The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene.
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑12 is immobilized at 0.5 µg/mL, 100 µL/well, the concentration of Recombinant Human IL‑12 R beta 2 Fc that produces 50% of the optimal binding response is 0.015-0.09 µg/mL.
• Molecular Mass: 95 kDa
• N-terminal Sequence Analysis: Lys24
• Endotoxin: <0.10 EU per 1 µg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose.
• Reconstitution: Reconstitute at 250 µg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.
• Publications:
  Human IL-35 Inhibits the Bioactivity of IL-12 and Its Interaction with IL-12Rβ2 (2023)

Gene Information

• Gene Name: IL12RB2 interleukin 12 receptor subunit beta 2 [ Homo sapiens (human) ]
• Official Symbol: IL12RB2
• Synonyms: IL12RB2; interleukin 12 receptor subunit beta 2; interleukin-12 receptor subunit beta-2; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; interleukin 12 receptor, beta 2; interleukin-12 receptor beta-2 chain
• Gene ID: 3595
• mRNA Refseq: NM_001559
• Protein Refseq: NP_001550
• MIM: 601642
• UniProt ID: Q99665