Active Recombinant Human IL11 protein, His-Avi-tagged, Biotinylated

• Cat.No.: IL11-1582H
• Product Overview: Biotinylated Recombinant Human IL11 protein(Pro22-Leu199), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 22-199 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4.
• Bio-activity: Immobilized Biotinylated Human IL-11, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5. μg/well) plate can bind Human IL-11 R alpha, Fc Tag with a linear range of 0.4-13 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 22.7 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Pro22-Leu199
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL11 interleukin 11 [ Homo sapiens ]
• Official Symbol: IL11
• Synonyms: IL11; interleukin 11; interleukin-11; adipogenesis inhibitory factor; AGIF; IL 11; oprelvekin; IL-11
• Gene ID: 3589
• mRNA Refseq: NM_000641
• Protein Refseq: NP_000632
• MIM: 147681
• UniProt ID: P20809