Active Recombinant Human IL10 protein, His-tagged

• Cat.No.: IL10-2627H
• Product Overview: Active Recombinant Human IL-10 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Ser19-Asn178) of human IL10/Interleukin-10 (Accession #NP_000563.1) fused with a 6×His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Ser19-Asn178
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1.Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is 0.13-0.52 ng/mL.
  2.Measured by its binding ability in a functional ELISA.Immobilized Human IL10 at 2 μg/mL (100 μL/well) can bind Human IL10RA with a linear range of 0.1-2.9 ng/mL.
• Molecular Mass: 18 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 92% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: IL10 interleukin 10 [ Homo sapiens ]
• Official Symbol: IL10
• Synonyms: IL10; interleukin 10; interleukin-10; CSIF; cytokine synthesis inhibitory factor; IL 10; IL10A; T cell growth inhibitory factor; TGIF; T-cell growth inhibitory factor; GVHDS; IL-10; MGC126450; MGC126451
• Gene ID: 3586
• mRNA Refseq: NM_000572
• Protein Refseq: NP_000563
• MIM: 124092
• UniProt ID: P22301