Active Recombinant Human IL10 Protein

• Cat.No.: IL10-060H
• Product Overview: The mature recombinant human IL-10 protein consists of 152 amino acids (19-178) without tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 19-178 a.a.
• Description: The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
• Bio-activity: Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is 0.15-0.75 ng/mL.
• Molecular Mass: 18.6 kDa
• Product-Related Proteins: 19-178
• Endotoxin: < 0.1 EU/μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: At -80 centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: PBS

Gene Information

• Gene Name: IL10 interleukin 10 [ Homo sapiens (human) ]
• Official Symbol: IL10
• Synonyms: IL10; interleukin 10; CSIF; TGIF; GVHDS; IL-10; IL10A; interleukin-10; T-cell growth inhibitory factor; cytokine synthesis inhibitory factor
• Gene ID: 3586
• mRNA Refseq: NM_000572
• Protein Refseq: NP_000563
• MIM: 124092
• UniProt ID: P22301