Active Recombinant Mouse Il10 Protein

• Cat.No.: Il10-083M
• Product Overview: Purified recombinant protein of Mouse interleukin 10 (Il10) (Ser19-Ser178) without tag was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 19-178 a.a.
• Description: This gene encodes an anti-inflammatory cytokine that is a member of the class-2 cytokine family. The encoded protein is secreted by cells of both the innate and adaptive immune systems and is crucial for limiting the immune response to a broad range of pathogens. It also has been shown to suppress autoimmune responses. This protein mediates it's immunosuppressive signal through a specific interleukin 10 receptor complex. Aberrant functioning of this gene is associated with numerous immune disorders including graft-versus-host disease, and increased susceptibility to HIV-1 infection and rheumatoid arthritis.
• Bio-activity: The ED50 is 1- 2 ng/ml, corresponding to a specific activity of 1- 0.5 x 10^6 units/mg, determined by the dose dependent stimulation MC/9 cell proliferation.
• Molecular Mass: 18.9 kDa
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Storage Buffer: 20mM Tris pH7.5

Gene Information

• Gene Name: Il10 interleukin 10 [ Mus musculus (house mouse) ]
• Official Symbol: Il10
• Synonyms: Il10; interleukin 10; CSIF; Il-10; interleukin-10; cytokine synthesis inhibitory factor
• Gene ID: 16153
• mRNA Refseq: NM_010548
• Protein Refseq: NP_034678
• UniProt ID: P18893