Active Recombinant Human IgG2 protein(Glu99-Lys327,257Ser/Ala)

• Cat.No.: IgG2-3405H
• Product Overview: Recombinant Human IgG2 Fc region (P01859) (Glu 99-Lys 327, 257Ser/Ala) was expressed and purified in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: Glu99-Lys327,257Ser/Ala
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind human CD32a-His in a functional ELISA.
• Molecular Mass: The recombinant human IgG2-Fc consists of 228 amino acids and has a predicted molecular mass of 25 kDa. As a result of glycosylation, the apparent molecular mass of IgG2-Fc is approximately 34 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.