Active Recombinant Human IgG1 protein, His-tagged

• Cat.No.: IgG1-1271H
• Product Overview: Recombinant Human IgG1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Pro100-Lys330) of human IgG1 Fc fused with a 6×His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Pro100-Lys330
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human Fc-gamma RII-a(CD32a) at 1 μg/mL (100 μL/well) can bind IgG1 Fc with a linear range of 0.156-3.47 μg/mL.
• Molecular Mass: 37kDa
• Endotoxin: <0.1EU/ug
• Purity: > 95% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80°C for 12 months.
  After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.