Active Recombinant Human IGF1 protein(Gly49-Ala118)

Cat.No. : IGF1-60H
Product Overview : Recombinant Human IGF1 (P05019-1) (Gly49-Ala118) was expressed in E. coli.
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Species : Human
Source : E.coli
Tag : Non
Protein Length : Gly49-Ala118
Form : Lyophilized from sterile 30% Acetonitrile, 0.1% TFA. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1.Measured by its binding ability in a functional ELISA. Immobilized Human IGF1 at 2 μg/ml (100 μl/well) can bind Human IGFBP4 His, the EC50 of Human IGFBP4 His is 10-60 ng/mL. 2. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 3.5-14 ng/mL.
Molecular Mass : The recombinant human IGF1 consists of 71 amino acids and has a calculated molecular mass of 7.8 KDa.
Purity : > 95 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name IGF1 insulin-like growth factor 1 (somatomedin C) [ Homo sapiens ]
Official Symbol IGF1
Synonyms IGF1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; IGF1A; MGF; IGF-IA; IGF-IB; somatomedin-C; mechano growth factor; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; IGFI; IGF-I;
Gene ID 3479
mRNA Refseq NM_000618
Protein Refseq NP_000609
MIM 147440
UniProt ID P05019

Not For Human Consumption!

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