Active Recombinant Human IFNG

• Cat.No.: IFNG-1367H
• Product Overview: Recombinant human IFN-γ produced in E. coli. is a non-covalent Dimer non-glycosylated, containing 140 amino acids, and having a molecular mass of approximately 16.5kDa.

Specification

• Description: Interferon-gamma (IFN-γ, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments TH1 cell expansion and may be required for TH1 cell differentiation. IFN-γ exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN-γ receptor is present on almost all cell types except mature erythrocytes and has been cloned and characterized. The IFN-γ receptor is structurally related to the recently cloned IL-10 receptor.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2μm filtered solution in 20mM Phosphate buffer
• Bio-activity: Bioactivity is detected using WISH cell (a heteroploid human amnion cell line). The specific activity shall be not less than 2.0×10^7 IU/mg of protein.
• Endotoxin: ≤1EU/μg, determined by the LAL method.
• Purity: ≥97%, as determined by SDS-PAGE and HPLC method.
• Usage: FOR RESEARCH ONLY
• Storage: Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2-8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 10μg/ml of the cytokine.
• Tag: Non
• Protein length: 140 a.a.

Gene Information

• Gene Name: IFNG interferon, gamma [ Homo sapiens ]
• Official Symbol: IFNG
• Synonyms: IFG; IFI
• Gene ID: 3458
• mRNA Refseq: NM_000619
• Protein Refseq: NP_000610
• MIM: 147570
• UniProt ID: P01579
• Chromosome Location: 12q14
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Allograft Rejection, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem
• Function: cytokine activity; interferon-gamma receptor binding