Active Recombinant Human IFNA4 protein, hFc-tagged

• Cat.No.: IFNA4-29H
• Product Overview: Recombinant Human IFNA4 protein(NP_066546.1)(Cys23-Asp189), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV).The EC50 for this effect is 0.2-1 ng/mL.
• Molecular Mass: The recombinant human Fc/IFN-α4a is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and has a predicted molecular mass of 46 kDa. As a result of glycosylation, the apparent molecular mass of rh Fc/IFN-α4a monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Cys23-Asp189
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 23-189 a.a.

Gene Information

• Gene Name: IFNA4 interferon, alpha 4 [ Homo sapiens ]
• Official Symbol: IFNA4
• Synonyms: IFNA4; interferon, alpha 4; interferon alpha-4; IFN alpha4a; MGC142200; IFN-alpha-4; interferon alpha-4B; interferon alpha-76; interferon alpha-M1; INFA4; IFN-alpha4a
• Gene ID: 3441
• mRNA Refseq: NM_021068
• Protein Refseq: NP_066546
• MIM: 147564
• UniProt ID: P05014