Active Recombinant Human IFNA2B Protein (165 aa)

• Cat.No.: IFNA2-106I
• Product Overview: Recombinant Human IFNA2B Protein without tag was expressed in Saccharomyces cerevisiae.

Specification

• Species: Human
• Source: S.Cerevisiae
• Protein Length: 165
• Description: At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: Fully biologically active when compared to standard. Fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay was found to be no less than 1.6 × 10^8 IU/mg.
• Molecular Mass: Approximately 19 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids
• AA Sequence: CDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
• Endotoxin: Less than 1 EU/μg of rHuIFN-a2b as determined by LAL method.
• Purity: >98% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable for several weeks at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20C. Further dilutions should be made in appropriate buffered solutions

Gene Information

• Gene Name: IFNA2 interferon alpha 2 [ Homo sapiens (human) ]
• Official Symbol: IFNA2
• Synonyms: IFNA2; interferon alpha 2; IFNA; IFNA2B; leIF A; IFN-alphaA; IFN-alpha-2; interferon alpha-2alpha-2a interferoninterferon alpha 2ainterferon alpha 2binterferon alpha A
• Gene ID: 3440
• mRNA Refseq: NM_000605
• Protein Refseq: NP_000596
• MIM: 147562
• UniProt ID: P01563