Active Recombinant Human IFNA1 protein, Fc-tagged

• Cat.No.: IFNA1-0257H
• Product Overview: Recombinant Human IFNA1 protein(Cys24-Glu189), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Human IFN-alpha / beta R1, His Tag on NTA Biosensor, can bind Human IFN-alpha 1, Fc Tag with an affinity constant of 11.3 nM as determined in BLI assay (ForteBio Octet Red96e).Loaded Human IFNAR2, His Tag on HIS1K Biosensor, can bind Human IFN-alpha 1, Fc Tag with an affinity constant of 2.67 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 45.8 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Cys24-Glu189
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 24-189 a.a.

Gene Information

• Gene Name: IFNA1 interferon, alpha 1 [ Homo sapiens ]
• Official Symbol: IFNA1
• Synonyms: IFNA1; interferon, alpha 1; interferon alpha-1/13; IFL; IFN; IFN ALPHA; IFN alpha 1b; IFN alphaD; IFNA13; IFNA@; interferon alpha 1b; leIF D; IFN-alpha 1b; IFN-alpha-1/13; interferon-alpha1; interferon alpha-D; IFN-ALPHA; IFN-alphaD; MGC138207; MGC138505; MGC138507
• Gene ID: 3439
• mRNA Refseq: NM_024013
• Protein Refseq: NP_076918
• MIM: 147660
• UniProt ID: P01562