Active Recombinant Human IDH1 Protein, His-tagged

• Cat.No.: IDH1-1044H
• Product Overview: Recombinant Human IDH1 (Accession # O75874) Met1-Leu414, fused with a C-terminal 6-His tag, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1-Leu414
• Predicted N Terminal: Ser2
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, Glycerol, Brij-35 and DTT.
• Bio-activity: Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate.The specific activity is >10,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human IDH1, His-tagged has a calculated MW of 47 kDa. In SDS-PAGE migrates as 43-45 kDa, reducing conditions..
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: IDH1 isocitrate dehydrogenase 1 (NADP+), soluble [ Homo sapiens ]
• Official Symbol: IDH1
• Synonyms: IDH1; isocitrate dehydrogenase 1 (NADP+), soluble; isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; oxalosuccinate decarboxylase; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; IDH; IDP; IDCD; IDPC; PICD
• Gene ID: 3417
• mRNA Refseq: NM_005896
• Protein Refseq: NP_005887
• MIM: 147700
• UniProt ID: O75874
• Chromosome Location: 2q32-qter