Active Recombinant Human ICOS protein, rFc-tagged

• Cat.No.: ICOS-3245H
• Product Overview: Recombinant Human ICOS protein(NP_036224.1)(Met1-Phe141), fused with rFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: rFc
• Protein Length: Met1-Phe141
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.5mM ETDA, pH 8.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized Recombinant Human ICOS Ligand / B7-H2 / ICOSLG Protein (ECD,His Tag)(Cat:11559-H08H) at 2 μg/ml (100 μl/well) can bind Recombinant Human ICOS / AILIM / CD278 Protein (Fc Tag)(Cat:10344-H31H),The EC50 is 40-120 ng/mL.
• Molecular Mass: The recombinant human ICOS consists of 345 amino acids and predicts a molecular mass of 38.9 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ICOS inducible T-cell co-stimulator [ Homo sapiens ]
• Official Symbol: ICOS
• Synonyms: ICOS; inducible T-cell co-stimulator; inducible T-cell costimulator; activation inducible lymphocyte immunomediatory molecule; AILIM; CD278; inducible costimulator; activation-inducible lymphocyte immunomediatory molecule; CVID1; MGC39850
• Gene ID: 29851
• mRNA Refseq: NM_012092
• Protein Refseq: NP_036224
• MIM: 604558
• UniProt ID: Q9Y6W8