Active Recombinant Human ICOS, Fc-His tagged

• Cat.No.: ICOS-3243H
• Product Overview: Recombinant Human ICOS (NP_036224.1) extracellular domain (Met 1-Phe 141), fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met 1-Phe 141
• Form: Lyophilized from sterile PBS, pH 7.4.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Recombinant Human ICOS Ligand / B7-H2 / ICOSLG Protein (Fc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Human ICOS / AILIM / CD278 Protein (His & Fc Tag),The EC50 is 8-30 ng/mL.
• Molecular Mass: The recombinant human ICOS/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 368 amino acids and predicts a molecular mass of 41.6 kDa. The apparent molecular mass of rhICOS/Fc monomer is approximately 50 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ICOS inducible T-cell co-stimulator [ Homo sapiens ]
• Official Symbol: ICOS
• Synonyms: ICOS; inducible T-cell co-stimulator; inducible T-cell costimulator; activation inducible lymphocyte immunomediatory molecule; AILIM; CD278; inducible costimulator; activation-inducible lymphocyte immunomediatory molecule; CVID1; MGC39850
• Gene ID: 29851
• mRNA Refseq: NM_012092
• Protein Refseq: NP_036224
• MIM: 604558
• UniProt ID: Q9Y6W8