Active Recombinant Human ICOS protein

• Cat.No.: ICOS-1628H
• Product Overview: Recombinant Human ICOS was expressed in NS0.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Non
• Form: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Bio-activity: The biological activity of Human ICOS was determined by the ability to inhibit human T cell proliferation induced by rhB7H2/Fc Chimera in the presence of antiCD3. Wang, S. et al. (2000) Blood 96:2808. The expected ED50 is 1 - 4 μg/ml in the presence of 3 μg/ml rhB7H2/Fc Chimera and antiCD3 immobilized at 20 ng/ml.
• Molecular Mass: The predicted molecular weight of Recombinant Human ICOS is Mr 40.3 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 50-60 kDa.
• AA Sequence: eingsanyem fifhnggvqi lckypdivqq fkmqllkggq ilcdltktkg sgntvsiksl kfchsqlsnn svsfflynld hshanyyfcn lsifdpppfk vtltggylhi yesqlccqlk fiegrmdpks cdkthtcppc papellggps vflfppkpkd tlmisrtpev tcvvvdvshe dpevkfnwyv dgvevhnakt kpreeqynst yrvvsvltvl hqdwlngkey kckvsnkalp apiektiska kgqprepqvy tlppsrdelt knqvsltclv kgfypsdiav ewesngqpen nykttppvld sdgsfflysk ltvdksrwqq gnvfscsvmh ealhnhytqk slslspgk
• Endotoxin: <1.0 EU/µg as determined by the LAL method
• Purity: >90% by SDS-PAGE and analyzed by silver stain.
• Storage: This lyophilized protein is stable for six to twelve months when stored desiccated at -20 centigrade to -70 centigrade. After aseptic reconstitution, this protein may be stored at 2 centigrade to 8 centigrade for one month or at -20 centigrade to -70 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Gene Information

• Gene Name: ICOS inducible T-cell co-stimulator [ Homo sapiens ]
• Official Symbol: ICOS
• Synonyms: ICOS; inducible T-cell co-stimulator; inducible T-cell costimulator; activation inducible lymphocyte immunomediatory molecule; AILIM; CD278; inducible costimulator; activation-inducible lymphocyte immunomediatory molecule; CVID1; MGC39850
• Gene ID: 29851
• mRNA Refseq: NM_012092
• Protein Refseq: NP_036224
• MIM: 604558
• UniProt ID: Q9Y6W8
• Chromosome Location: 2q33
• Pathway: Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem