Active Recombinant Human ICAM2, Fc Chimera

• Cat.No.: ICAM2-476H
• Product Overview: Recombinant Human ICAM2 (Accession # CAA33630) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Lys25
• Form: Lyophilized from a 0.2 µm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.When 5 x 10^4 cells/well are added to rhICAM-2/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >70% will adhere after 1 hour incubation at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human ICAM2, Fc Chimera has a calculated MW of 50 kDa (monomer). In SDS-PAGE migrates as 86 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ICAM2 intercellular adhesion molecule 2 [ Homo sapiens ]
• Official Symbol: ICAM2
• Synonyms: ICAM2; intercellular adhesion molecule 2; CD102; ICAM-2
• Gene ID: 3384
• mRNA Refseq: NM_000873
• Protein Refseq: NP_000864
• MIM: 146630
• UniProt ID: P13598
• Chromosome Location: 17q23-q25