Active Recombinant Human IBSP Protein, His-tagged

• Cat.No.: IBSP-16H
• Product Overview: Recombinant Human IBSP Protein, fused to His-tag was expressed in HEK293T.

Specification

• Description: The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
• Source: HEK293T
• Species: Human
• Tag: His
• Form: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MCF7 human breast cancer cells. When cells are added to Human IBSP coated plates 3 ug/ml. This effect is more to 40%.
• Molecular Mass: 34.3 kDa
• Protein length: 17-317aa
• AA Sequence: FSMKNLHRRV KIEDSEENGV FKYRPRYYLY KHAYFYPHLK RFPVQGSSDS SEENGDDSSE EEEEEEETSN EGENNEESNE DEDSEAENTT LSATTLGYGE DATPGTGYTG LAAIQLPKKA GDITNKATKE KESDEEEEEE EEGNENEESE AEVDENEQGI NGTSTNSTEA ENGNGSSGGD NGEEGEEESV TGANAEDTTE TGRQGKGTSK TTTSPNGGFE PTTPPQVYRT TSPPFGKTTT VEYEGEYEYT GANEYDNGYE IYESENGEPR GDNYRAYEDE YSYFKGQGYD GYDGQNYYHH Q
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm).

Gene Information

• Gene Name: IBSP integrin binding sialoprotein [ Homo sapiens (human) ]
• Official Symbol: IBSP
• Synonyms: BSP; BNSP; SP-II; BSP-II
• Gene ID: 3381
• mRNA Refseq: NM_004967.4
• Protein Refseq: NP_004958.2
• MIM: 147563
• UniProt ID: P21815