Active Recombinant Human HYAL1 protein, His-tagged

• Cat.No.: HYAL1-13H
• Product Overview: Recombinant Human HYAL1(Met1-Trp435) fused with His tag at C-terminal was expressed in CHO.

Specification

• Description: Human hyaluronidases (HYALs) are a group of five endo‑ beta ‑N‑acetyl‑hexosaminidases that include HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20). While HYAL1, HYAL2 and SPAM1 are endo‑ beta ‑N‑acetyl‑glucosaminidase that are mainly active on hyaluronan with little activity on chondroitin sulfate, HYAL4 is mainly active on chondroitin sulfate (type C and D in particular). Hyaluronan and chondroitin sulfate are abundant extracellular matrix components that have numerous biological function. The backbone of chondroitin sulfate composed of repeating units of ‑4GlcA beta 1‑3GalNAc beta 1‑ most closely resembles to that of hyaluronan composed of repeating units of ‑4GlcA beta 1‑3GlcNAc beta 1‑, which may partially explain the overlapping substrate specificity of most hyaluronidases. HYAL1 is a lysosomal hyaluronidase with optimal activity around pH 4.0. It is highly expressed in the liver, kidney and heart, and is the predominant hyaluronidase found in plasma. Defects in HYAL1 are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. Surprisingly, HYAL1 has been reported both as a tumor promoter and suppressor.The enzymatic activity of recombinant human HYAL1 was measured using 35S‑labeled hyaluronan as substrate.
• Source: CHO
• Species: Human
• Tag: His
• Predicted N Terminal: Phe22
• Form: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to hydrolyze S-35 labeled hyaluronan. <20 ng of rhHYAL1 is required for the 50% hydrolysis of 10 μg hyaluronan.
• Molecular Mass: Predicted Molecular Mass: 47 kDa;
  SDS-PAGE: 50-60 kDa, reducing conditions
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
• Storage: Avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Protein length: 1-435 a.a.

Gene Information

• Gene Name: HYAL1 hyaluronoglucosaminidase 1 [ Homo sapiens ]
• Official Symbol: HYAL1
• Synonyms: HYAL1; hyaluronoglucosaminidase 1; hyaluronidase-1; FUS2; HYAL 1; LUCA1; NAT6; luCa-1; hyaluronidase 1; plasma hyaluronidase; tumor suppressor LUCA-1; lung carcinoma protein 1; hyaluronoglucosaminidase-1; HYAL-1; MGC45987
• Gene ID: 3373
• mRNA Refseq: NM_007312
• Protein Refseq: NP_009296
• MIM: 607071
• UniProt ID: Q12794