Active Recombinant Human HS3ST3B1, His-tagged

• Cat.No.: HS3ST3B1-118H
• Product Overview: Chinese Hamster Ovary cell line, CHO-derived, Ser54-Asp390 with an N-terminal CD33 signal sequence and a C-terminal 6-His tag

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 54-390 a.a.
• Description: Human Heparan Sulfate Glucosamine 3?O?sulfotransferase 3 belongs to the family of transferases, specifically the sulfotransferases, which transfer sulfur-containing groups. The systematic name of this enzyme class is 3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine 3-sulfotransferase. Other names in common use include heparin-glucosamine 3-O-sulfotransferase, 3'-phosphoadenylyl-sulfate:heparin-glucosamine 3-O-sulfotransferase, glucosaminyl 3-O-sulfotransferase, heparan sulfate D-glucosaminyl 3-O-sulfotransferase, and isoform/isozyme 1 (3-OST-1, HS3ST1). This enzyme participates in heparan sulfate biosynthesis and glycan structures - biosynthesis 1.
• Predicted N Terminal: Gly60
• Form: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Brij-35.
• Bio-activity: Measured by its ability to transfer sulfate from PAPS to heparan sulfate.The specific activity is >25 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: 38 kDa, 39-52 kDa, reducing SDS-PAGE conditions.
• Endotoxin: < 1.0 eu per 1 μg of the protein by the lal method
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie? Blue stain at 5 μg per lane.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 °C as supplied.3 months, -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: HS3ST3B1 heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1 [ Homo sapiens ]
• Official Symbol: HS3ST3B1
• Synonyms: HS3ST3B1; heparan sulfate (glucosamine) 3-O-sulfotransferase 3B1; heparan sulfate glucosamine 3-O-sulfotransferase 3B1; 3OST3B1; 30ST3B1; 3-OST-3B; h3-OST-3B; heparan sulfate 3-O-sulfotransferase 3B1; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3B1
• Gene ID: 9953
• mRNA Refseq: NM_006041
• Protein Refseq: NP_006032
• MIM: 604058
• UniProt ID: Q9Y662
• Chromosome Location: 17p12
• Pathway: Glycosaminoglycan biosynthesis - heparan sulfate, organism-specific biosystem; Glycosaminoglycan biosynthesis - heparan sulfate, conserved biosystem; heparan sulfate biosynthesis, organism-specific biosystem; heparan sulfate biosynthesis, conserved biosystem; heparan sulfate biosynthesis (late stages), organism-specific biosystem; heparan sulfate biosynthesis (late stages), conserved biosystem
• Function: [heparan sulfate]-glucosamine 3-sulfotransferase 1 activity; [heparan sulfate]-glucosamine 3-sulfotransferase 3 activity; sulfotransferase activity; transferase activity