Active Recombinant Human HPN Protein, His-tagged

• Cat.No.: HPN-148H
• Product Overview: Recombinant Human HPN Protein(P05981)(Arg45-Leu417), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22μm filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human HPN, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 20.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 42 kDa. Due to the protein cleaved into the following two chains, so it migrates to 18-22 kDa and 30 kDa based on Tris-Bis PAGE result.
• Protein length: Arg45-Leu417
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -20 to -80°C for 3-6 months in unopened state after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).

Gene Information

• Gene Name: HPN hepsin [ Homo sapiens ]
• Official Symbol: HPN
• Synonyms: HPN; hepsin; serine protease hepsin; TMPRSS1; transmembrane protease; serine 1; transmembrane protease serine 1; transmembrane protease, serine 1; serine protease hepsin catalytic chain; serine protease hepsin non-catalytic chain
• Gene ID: 3249
• mRNA Refseq: NM_002151
• Protein Refseq: NP_002142
• MIM: 142440
• UniProt ID: P05981