Active Recombinant Human HDAC6 Protein

• Cat.No.: HDAC6-313H
• Product Overview: Recombinant Human HDAC6 Protein is produced by HEK293 cells expression system.

Specification

• Description: HDAC6 is a Class II HDAC. It is unique among human HDACs in having two full deacetylase domains. As a tubulin deacetylase, HDAC6 may act to promote autophagic clearing of Huntington aggregates and retard HIV1 infection. It can shuttle between the nucleus and cytoplasm, suggesting potential extra nuclear functions by regulating the acetylation status of non-histone substrates. By modifying chromatin structure and other non-histone proteins, HDACs play important roles in controlling complex biological events, including cell development, differentiation, programmed cell death, angiogenesis, and inflammation. Considering these major roles, it is conceivable that dysregulation of HDACs and subsequent imbalance of acetylation and deacetylation may be involved in the pathogenesis of various diseases, including cancer and inflammatory diseases.
• Source: HEK293 cells
• Species: Human
• Form: Liquid, In 30 mM HEPES, 140 mM NaCl, 10 mM KCl, 3% glycerol, 0.25 mM TCEP, pH 7.4.
• Bio-activity: Deacetylation activity was determined using GAK(Ac)-AMC, Boc-K(Ac)-AMC and Fluor-de-Lys (RHK-K(Ac)-AMC) substrates. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and a fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by non-linear regression analysis.
• Molecular Mass: 131 kDa
• Protein length: amino acids 2–1215
• Purity: ≥90% by SDS-PAGE
• Notes: For Research Use Only! Not For Use in Humans.
• Storage: Store at -80 centigrade.
• Handling: Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles.
• Tag: Non

Gene Information

• Gene Name: HDAC6 histone deacetylase 6 [ Homo sapiens ]
• Official Symbol: HDAC6
• Synonyms: HDAC6; histone deacetylase 6; FLJ16239; HD6; JM21; KIAA0901
• Gene ID: 10013
• mRNA Refseq: NM_006044
• Protein Refseq: NP_006035
• MIM: 300272
• UniProt ID: Q9UBN7

Reviews

06/23/2019

Their expertise and guidance have helped overcome hurdles, ensuring the smooth progress of my research.

12/07/2018

The bands of proteins in Western Blotting experiments were very clear.

09/16/2018

Its purity and consistency have provided me with reliable and reproducible results, ensuring the accuracy of my research findings.

Q&As

Can HDAC6 inhibitors be used in combination with other therapies, and if so, what synergistic effects are expected?

Yes, HDAC6 inhibitors can be combined with other therapies for enhanced efficacy. Synergistic effects may include improved anti-cancer activity or neuroprotection.

Are there any ongoing clinical trials targeting HDAC6, and if so, what are the potential outcomes for patients?

Yes, there are clinical trials exploring HDAC6 inhibitors as potential treatments. Positive outcomes could lead to new therapeutic options for patients.

In what other diseases has HDAC6 been implicated, and how might this knowledge be applied clinically?

HDAC6 is implicated in neurodegenerative diseases and inflammatory disorders. Understanding its role could lead to novel therapeutic approaches for these conditions.

How does HDAC6 influence cellular processes, and why is this relevant in a clinical setting?

HDAC6 regulates cell motility, protein trafficking, and other processes. Understanding these mechanisms is crucial for developing targeted therapies in various diseases.

Are there any known genetic variations in HDAC6, and how might these impact individual responses to treatments in a clinical setting?

Genetic variations in HDAC6 may influence individual responses to treatments. Personalized medicine approaches could consider these variations for better outcomes.